Perpetual Taq PCR Master Mix (2x)

Description:

• Perpetual Taq PCR Master Mix (2x) is a ready-to-use solution containing Perpetual Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
• Use of Perpetual Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR setup.
• Perpetual Taq DNA Polymerase contains recombinant Taq DNA Polymerase bound to an anti-Taq monoclonal antibody that blocks polymerase activity at moderate temperatures.
• Anti-Taq antibodies inhibit polymerase activity at temperatures up to 70°C.
• The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes.
• Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "hot start" PCR, which allows for convenient room-temperature reaction setup.
• "Hot start" PCR may increase specificity, sensitivity and yield of a PCR reaction in comparison to the conventional PCR assembly method.
• Perpetual Taq DNA Polymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C (1,2).
• Contains the 5'->3' exonuclease activity.
• Lacks the 3'->5' exonuclease activity.
• Adds extra A at the 3' ends.
• Perpetual Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10 μg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 μl.

Storage Conditions: Store at -20°C for long-term storage or at 4°C for up to 2 months.

Perpetual Taq PCR Master Mix (2x) contains:

1. Perpetual Taq PCR Master Mix (2x)
2. Water, nuclease free
3. 10 x Color Load

Perpetual Taq PCR Master Mix (2x): Perpetual Taq DNA Polymerase is supplied in 2 x Pol Buffer B containing 3 mM MgCl2 and 0.4 mM of each dNTP..

10 x Color Load: contains two gel tracking dyes and a gel loading reagent. It enables direct loading of PCR products onto an agarose gel..

Quality Control: All preparations are assayed for contaminating endonuclease, 3-exonuclease, and non-specific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644.

Description:

• Perpetual Taq PCR Master Mix (2x) is a ready-to-use solution containing Perpetual Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
• Use of Perpetual Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR setup.
• Perpetual Taq DNA Polymerase contains recombinant Taq DNA Polymerase bound to an anti-Taq monoclonal antibody that blocks polymerase activity at moderate temperatures.
• Anti-Taq antibodies inhibit polymerase activity at temperatures up to 70°C.
• The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes.
• Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "hot start" PCR, which allows for convenient room-temperature reaction setup.
• "Hot start" PCR may increase specificity, sensitivity and yield of a PCR reaction in comparison to the conventional PCR assembly method.
• Perpetual Taq DNA Polymerase replicates DNA at 72°C and exhibits a half-life of 40 min at 95°C (1,2).
• Contains the 5'->3' exonuclease activity.
• Lacks the 3'->5' exonuclease activity.
• Adds extra A at the 3' ends.
• Perpetual Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10 μg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 μl.

Storage Conditions: Store at -20°C for long-term storage or at 4°C for up to 2 months.

Perpetual Taq PCR Master Mix (2x) contains:

1. Perpetual Taq PCR Master Mix (2x)
2. Water, nuclease free
3. 10 x Color Load

Perpetual Taq PCR Master Mix (2x): Perpetual Taq DNA Polymerase is supplied in 2 x Pol Buffer B containing 3 mM MgCl2 and 0.4 mM of each dNTP..

10 x Color Load: contains two gel tracking dyes and a gel loading reagent. It enables direct loading of PCR products onto an agarose gel..

Quality Control: All preparations are assayed for contaminating endonuclease, 3-exonuclease, and non-specific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644.