GeneMATRIX PLASMID MINIPREP DNA Purification Kit (Kit for isolation of high-purity plasmid DNA (1.5-4 ml bacterial culture).

GeneMATRIX Plasmid Miniprep DNA Purification Kit is designed to isolate very high purity plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains. Plasmid DNA contaminants such as: RNA, single-stranded DNA, enzymes/proteins, lipids, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed from crude bacterial lysate. The membranes used for construction of the Plasmid Miniprep Kit is especially optimized toward removal of problematic inhibitors of restriction of DNA as well as non-specific endo- and exonucleases. Coloured lysis buffer helps both in monitoring cell solubilization progress as well as simultaneous processing of multiple samples. Optimized buffer is added to provide selective conditions for DNA binding to the GeneMATRIX during brief centrifugation, while contaminants pass through the spin-column. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps. High-quality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Izolated DNA is ready for downstream applications without the need for ethanol precipitation.