HOT FIREPol DNA Polymerase

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HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase.

At ambient temperatures it is inactive, having no polymerization activity.

HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C.

This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→5’ exonuclease activity.