5x HOT FIREPol® EvaGreen®qPCR Supermix

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only 

Description:

HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises all the components necessary to perform qPCR excluding the template and primers, to perform highly sensitive qPCR.

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Mix compatibility

Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™,Chromo4™, Opticon® 2 & MiniOpticon®
Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
Eppendorf: Mastercycler®: ep realplex2 & ep 
realplex4
Illumina: The Eco™ 
Roche: LightCycler® 480

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