HOT FIREPol Probe Universal qPCR Mix

5x HOT FIREPol® Probe GC qPCR Mix

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only 

Description:

5x HOT FIREPol® Probe GC qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables efficient amplification of GC-rich (up to 75%) targets. HOT FIREPol® Probe GC qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5´–3´ exonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup