BM Labosis'e Hoşgeldiniz

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DdeI, (DdeI)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
DdeI, (DdeI)
E2140-01
500 u 80 EUR
+%20 KDV
3.552,00 TL
DdeI, (DdeI)
E2140-02
2 500 u 320 EUR
+%20 KDV
14.208,00 TL

DdeI

(Desulfovibrio desulfuricans)

5’-C T N A G-3’
3’-G A N T C-5’

Reaction Temperature: 37°C

Prototype: DdeI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x High Buffer: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl,1 mM dithiothreitol, 100 µg/ml bovine serum albumin.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

Assay Conditions: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin, 1 µg of lambda DNA. Incubation is at 37°C for 1 hr in a reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control: All preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5'-exonuclease/5'-phosphatase, for nickase as well as for nonspecific single- and double-stranded DNase activities.

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