First Strand cDNA Sentez Kiti (First Strand cdna Synthesis Kit)

First Strand cdna Synthesis Kit Application:

• cDNA synthesis
• RNA analysis by primer extension
• DNA labeling

First Strand cdna Synthesis Kit Reagents Provided:

• M-MLV Reverse Transcriptase RNase H- 2000U
• 5x RT Reaction buffer 1 (with MgCl2 and DTT) 0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
• 5x RT Reaction buffer 2 (Mg2+ and DTT free) 0.25 M Tris-HCl, 0.5 M KCl
• 25 mM MgCl2 · 20 mM MnCl2
• Oligo (dt) 50µM 15µL
• Random Hexamer 50µM 15µL
• dNTP Mix 20mM 15µL
• RNase Inhibitor 8μl 5mg/mL