BM Labosis'e Hoşgeldiniz

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Random Hexamer Primers

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Random Hexamer Primers
E0101-01
20 μg 40 EUR
+%20 KDV
1.776,00 TL

 

Description:
Random Hexamer Primer is a mixture of single-stranded random
hexonucleotides with 5' and 3' – hydroxyl ends. Random Hexamer
Primers are used for priming single-stranded DNA or RNA by DNA
polymerases or reverse transcriptases.
 
Primer sequence:
5'- d (NNNNNN) – 3'
N=G, A, T, C
Concentration:
500 ng/μl, 250 μM, 025 nmol/μl.
 
Application:
• cDNA synthesis using reverse transcriptase with RNA template*;
• in situ hybridization, Southern, Nothern probe synthesis (1);
• DNA synthesis using Klenow fragment with DNA template;
• radiolabeling of DNA probe (2).
*Note: for reverse transcription use 100-300 ng per 20 μl reaction.
 
Quality Control:
Assayed for nucleases and non specific RNA and DNA contamination.
 
References:
1. Fisk, F.Z and Hodgson, C.P (1987) Nucl. Acid Res., 15, 6295.
2. Feinberg, A.P. and Vogalstein, B. (1983) Analytical Biochem. 132, 6-13.
 
 
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