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AvaI, (AvaI)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
AvaI, (AvaI)
E2006-01
2 000 u 57,6 EUR
+%20 KDV
2.833,92 TL
AvaII, (AvaII)
E2007-01
1 000u 62,4 EUR
+%20 KDV
3.070,08 TL
AvaI, (AvaI)
E2006-02
10 000 u 228,8 EUR
+%20 KDV
11.256,96 TL
AvaII, (AvaII)
E2007-02
5 000u 249,6 EUR
+%20 KDV
12.280,32 TL

AvaI

(Anabaena variabilis)

5’-C Py C G Pu G -3’
3’-G Pu G C Py C -5’

Reaction Temperature: 37°C

Prototype: AvaI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x Medium I Buffer: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

Assay Conditions: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin and 1 µg of lambda DNA. Incubation is at 37°C for 1 hr in a reaction volume of 50 µl.

Storage Buffer: 10 mM Tris-HCl (pH 7.4 at 22°C), 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA and 50% (v/v) glycerol.

Storage Conditions: Store at –20oC.

Quality Control:

Non-specific Endonuclease: Incubation of 10 units of AvaI with 1 µg of lambda DNA at 37°C for 16 hrs (a 160-fold over-digestion) resulted in the same sharp characteristic banding pattern as the standard assay reaction, as determined by agarose gel electrophoresis.

3'-Exonuclease: 5, 10 and 20 units of AvaI and 0.13 µg of lambda/TaqI fragments (3'-labeled with T4 DNA Polymerase and [³H]dGTP and [³H]dCTP), incubated for 1 hr at 37°C resulted in 0.01-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

5'-Exonuclease/5'-Phosphatase: Incubation of 5, 10 and 20 units of AvaI with 0.05 µg of [5'-³²P] lambda/HaeIII fragments for 1 hr at 37°C resulted in 0.08-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

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