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Ovation® 3'-DGE System

Marka:Nugen

Ürün Ölçü Fiyat TL Miktar
Ovation® 3-DGE System
7200-08
8 Reac. 0 USD
+%20 KDV
0,00 TL

 

Ovation® 3'-DGE System

The Ovation 3'-DGE System provides accurate expression analysis (tag profiling) suitable for quantification of any poly(A)+ transcripts, without the need for prior sequence knowledge. It employs a simple, fast and automatable workflow, powered by the Ribo-SPIA® Single Primer Isothermal Amplification technology and is compatible with all leading NGS platforms.

The input for the Ovation 3'-DGE System is flexible. You can choose one of the two options that best fits with your experimental design:

  • 10 ng – 100 ng of purified total RNA
  • Direct cell lysate of 50 – 10,000 cells

The protocol for the Ovation 3'-DGE System takes only six hours to complete and generates micrograms of double-stranded cDNA, focusing on the most 3' 150 – 500 bases proximal to the poly(A) tail of the transcripts. The cDNA is suitable as input for any NGS library preparation kit, such as Encore® Rapid or Ovation Ultralow Library Systems.

DGE libraries can be produced in nine hours, greatly simplifying the entire sample preparation procedure. The combination of the Ovation 3'-DGE System and NuGEN's NGS Library Systems provides a complete, streamlined and easily automatable sample preparation workflow, enabling NGS-based expression profiling approaching the protocol simplicity and sample throughput of microarrays.

Figure 1. Simple and Fast Ovation 3'-DGE Workflow for Integrated NGS Analysis

Figure 2. Highly Concordant Differential Expression Results Obtained Comparing DGE and qPCR

 

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